Western Blot Analysis of EMT Signaling

Western blot analysis was performed as previously described by us [23 (link)]. The following antibodies were used in the analysis: anti-EVI5 (Millipore, Billerica, MA, USA); anti-Emi1 and anti-TGF-β receptor II (Santa Cruz, CA, USA); anti-CyclinA2 (Proteintech, IL, USA); anti-pAkt (Ser473), anti-Akt, anti-Erk1/2, anti-pErk (Thr202/Tyr204), anti-CyclinD1, anti-MMP2, anti-p-Smad3, anti-Snail and anti-β-actin (Cell Signaling Technology, Danvers, MA, USA); anti-TGF-β receptor I (Abcam, London, UK); anti-N-cadherin and anti-Vimentin (BD Biosciences, USA); Anti-mouse and anti-rabbit secondary antibodies (Cell Signaling Technology, Danvers, MA, USA).

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Cai T., Zhou J., Zeng Y., Du W., Zhang Y., Liu T., Fu Y., Huang J.A., Qian Q., Zhu J., Ling C, & Liu Z. (2020). EVI5 is an oncogene that regulates the proliferation and metastasis of NSCLC cells. Journal of Experimental & Clinical Cancer Research : CR, 39, 84.

Publication 2020

anti-Emi1 anti-TGF-β receptor II anti-CyclinA2 anti-Erk1/2 anti-pErk (Thr202/Tyr204), anti-CyclinD1 anti-MMP2 anti-p-Smad3 anti-Snail anti-β-actin anti-TGF-β receptor I anti-N-cadherin anti-Vimentin Anti-mouse and anti-rabbit secondary antibodies

Actin Anti antibodies Antibodies Cadherin Erk1 Mmp2 Mouse Rabbit Smad3 Snail Tgf β receptor i Tgf β receptor ii Vimentin Western blot analysis

Corresponding Organization :

Other organizations : Soochow University, First Affiliated Hospital of Soochow University, National Jewish Health, University of Colorado Denver, Suzhou Research Institute

Top 5 similar protocols

Variable analysis

independent variables

Anti-EVI5 (Millipore, Billerica, MA, USA)
Anti-Emi1 (Santa Cruz, CA, USA)
Anti-TGF-β receptor II (Santa Cruz, CA, USA)
Anti-CyclinA2 (Proteintech, IL, USA)
Anti-pAkt (Ser473) (Cell Signaling Technology, Danvers, MA, USA)
Anti-Akt (Cell Signaling Technology, Danvers, MA, USA)
Anti-Erk1/2 (Cell Signaling Technology, Danvers, MA, USA)
Anti-pErk (Thr202/Tyr204) (Cell Signaling Technology, Danvers, MA, USA)
Anti-CyclinD1 (Cell Signaling Technology, Danvers, MA, USA)
Anti-MMP2 (Cell Signaling Technology, Danvers, MA, USA)
Anti-p-Smad3 (Cell Signaling Technology, Danvers, MA, USA)
Anti-Snail (Cell Signaling Technology, Danvers, MA, USA)
Anti-TGF-β receptor I (Abcam, London, UK)
Anti-N-cadherin (BD Biosciences, USA)
Anti-Vimentin (BD Biosciences, USA)
Anti-mouse and anti-rabbit secondary antibodies (Cell Signaling Technology, Danvers, MA, USA)

dependent variables

EVI5 expression
Emi1 expression
TGF-β receptor II expression
CyclinA2 expression
PAkt (Ser473) expression
Akt expression
Erk1/2 expression
PErk (Thr202/Tyr204) expression
CyclinD1 expression
MMP2 expression
P-Smad3 expression
Snail expression
TGF-β receptor I expression
N-cadherin expression
Vimentin expression

control variables

Anti-β-actin (Cell Signaling Technology, Danvers, MA, USA)

Annotations

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