Biospecimen Collection for Genomic Analysis

At each 6-month visit, blood was collected: One gray-top tube was sent for drugs-of-abuse screening (~10 mL) and four purple-top BD vacutainer tubes containing K2-EDTA as the anticoagulant (Becton Dickinson & Co., Franklin Lakes, NJ) were used to collect blood from patients (~40 mL) for serum and PBMC isolation, as described [41 (link), 42 (link)]. From 5×10⁶ PBMCs, genomic DNA and total RNA isolation was performed using a Qiagen (Venlo, Limburg, Netherlands) AllPrep DNA/RNA procedure as described by the manufacturer.

Free full text: Click here

Nonnemacher M.R., Pirrone V., Feng R., Moldover B., Passic S., Aiamkitsumrit B., Dampier W., Wojno A., Kilareski E., Blakey B., Ku T.S., Shah S., Sullivan N.T., Jacobson J.M, & Wigdahl B. (2016). HIV-1 Promoter Single Nucleotide Polymorphisms Are Associated with Clinical Disease Severity. PLoS ONE, 11(4), e0150835.

Publication 2016

BD vacutainer tubes K2-EDTA AllPrep DNA/RNA procedure

Anticoagulant Blood Edta Genomic Isolation Patients Serum

Corresponding Organization : Drexel University

Other organizations : University of Pennsylvania

Top 5 similar protocols

Variable analysis

independent variables

None explicitly mentioned

dependent variables

Serum
PBMC isolation
Genomic DNA
Total RNA isolation

control variables

Gray-top tube for drugs-of-abuse screening
Four purple-top BD vacutainer tubes containing K2-EDTA as the anticoagulant

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!