SCFA Quantification by GC-FID-MS

Short-chain fatty acids (SCFA) were analyzed by gas chromatography (GC). Cell-free supernatants from fecal homogenates were mixed with methanol, 20% (v/v) formic acid, and an internal standard solution (2-ethylbutyric) in a 38:46:8:8 (v/v) proportion. This mixture was used for SCFA quantification in a system composed of a 6890NGC injection module (Agilent Technologies Inc., Palo Alto, CA, USA) connected to a flame injection detector (FID) and a mass spectrometry (MS) 5973N detector (Agilent), as described elsewhere [24 (link)].

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Arboleya S., Saturio S., Suárez M., Fernández N., Mancabelli L., de los Reyes-Gavilán C.G., Ventura M., Solís G, & Gueimonde M. (2020). Donated Human Milk as a Determinant Factor for the Gut Bifidobacterial Ecology in Premature Babies. Microorganisms, 8(5), 760.

Publication 2020

6890NGC injection module ) 5973N detector

Cell Fecal Formic acid Gas chromatography Mass spectrometry Methanol Short chain fatty acids

Corresponding Organization : Instituto de Productos Lácteos de Asturias

Other organizations : Central University Hospital of Asturias, University of Parma

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Variable analysis

independent variables

Proportions of methanol, 20% (v/v) formic acid, and internal standard solution (2-ethylbutyric)

dependent variables

Concentrations of short-chain fatty acids (SCFAs)

control variables

Cell-free supernatants from fecal homogenates
Gas chromatography (GC) system composed of a 6890NGC injection module, a flame injection detector (FID), and a mass spectrometry (MS) 5973N detector

controls

Internal standard solution (2-ethylbutyric)

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