For morphological characterization, specimens were initially observed with a Leica 205C stereomicroscope (Leica Biosystems, Nussloch GmbH, Nussloch, Germany). The microscopical characteristics were analyzed by mounting asexual structures in clear lactoglycerol, and 50 measurements for each morphological parameter were carried out at a magnification of × 1,000 using a Leica DM2500 light microscope equipped with a Leica DFC 490 digital camera, coupled to a computer containing the Leica Qwin-Plus software. The morphological characteristics of the isolates were compared with the description of R. gossypii and R. pseudoglycines14 ,15 (link).
For examination on a scanning electron microscope (JOEL JSM-700 1F model), fragments of symptomatic dry leaves were fixed in 10 mm diameter copper stubs with double-sided carbon tape and coated with 25 mA gold, 1.10–2 mbar, for 2.5 min.
For examination on a scanning electron microscope (JOEL JSM-700 1F model), fragments of symptomatic dry leaves were fixed in 10 mm diameter copper stubs with double-sided carbon tape and coated with 25 mA gold, 1.10–2 mbar, for 2.5 min.
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