To quantify cell growth, background hindrance was quantified in culture medium per well as described earlier [32 (link)]. Later HCT-116, SNU-C2A, and HT-29 cells were seeded on 16-well E-plates and incubated in Roche xCELLigence Real-Time Cell Analyzer (RTCA) DP instrument (Roche Diagnostics GmbH, Germany). TMP (10 μM)- and TGFβ (10 ng/mL)-treated cell index values were measured every 15 min time intervals.
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