Polyamine Quantification in Plant Leaves

A 0.1 g of leaves was homogenized with 1 mL of HClO₄ (5%, w/v), kept in an ice bath for 1 h, and then centrifuged at 12,000 rpm (Centrifuge 5425/5425 R; Eppendorf, Germany) for 30 min at 4 °C. The supernatant was stored at –70 °C for PAs concentration measurements by high-performance liquid chromatography (HPLC; Shimadzu LCMS8040 system, China) [11 (link)]. Standard Put (CAS No.: 333–93–7), Spm (CAS No.: 71–44–3), and Spd (CAS No.: 334–50–9) were purchased from Sigma–Aldrich (USA) and were used to optimize the mass spectrometric parameters and fragment spectra [12 (link),13 (link),14 (link)].

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Zhao X., Zhao C., Niu Y., Chao W., He W., Wang Y., Mao T, & Bai X. (2022). Understanding and Comprehensive Evaluation of Cold Resistance in the Seedlings of Multiple Maize Genotypes. Plants, 11(14), 1881.

Publication 2022

Centrifuge 5425/5425 R LCMS8040 system

Bath Hplc Mass spectrometric

Corresponding Organization :

Other organizations : Gansu Agricultural University, Wells Fargo (United States), United States Department of Agriculture

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Variable analysis

independent variables

Amount of leaves (0.1 g)

dependent variables

PA concentration measurements by HPLC

control variables

HClO4 concentration (5%, w/v)
Incubation time (1 h)
Centrifugation speed (12,000 rpm)
Centrifugation temperature (4 °C)
Storage temperature (-70 °C)

positive controls

Standard Put (CAS No.: 333-93-7)
Standard Spm (CAS No.: 71-44-3)
Standard Spd (CAS No.: 334-50-9)

negative controls

No information provided

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