Immortalized Mouse Lung Endothelial Cells

The mouse lung endothelial cell line and the mouse dEC line were generated by immortalizing primary cells isolated from adult C57/BL6 mice using SV40 large T-antigen^{11 (link),30 (link)–33 (link)}. The endothelial cell lines were cultured in DMEM with 10% FBS, penicillin–Streptomycin (100 µ/ml and 100 μg/ml, respectively) in a humidified cell culture incubator set at 5% CO₂ and 37 °C. The primary HUVECs from ATCC (PCS-100-013) were cultured with the Endothelial Cell Growth Kit-BBE (ATCC® PCS-100-040) following the kit`s instruction.

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Xiao W., Pinilla-Baquero A., Faulkner J., Song X., Prabhakar P., Qiu H., Moremen K.W., Ludwig A., Dempsey P.J., Azadi P, & Wang L. (2022). Robo4 is constitutively shed by ADAMs from endothelial cells and the shed Robo4 functions to inhibit Slit3-induced angiogenesis. Scientific Reports, 12, 4352.

Publication 2022

Endothelial Cell Growth Kit-BBE

Adult Cell culture Cell lines Cells Cultured cell Endothelial Endothelial cell Lung Mice Penicillin Streptomycin Sv40

Corresponding Organization :

Other organizations : USF Health Byrd Alzheimer's Institute, University of South Florida, University of Georgia, RWTH Aachen University, University of Colorado Denver

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Variable analysis

independent variables

Immortalization of primary cells using SV40 large T-antigen

dependent variables

Growth and viability of mouse lung endothelial cell line
Growth and viability of mouse dEC line

control variables

Culture conditions (DMEM with 10% FBS, penicillin-Streptomycin, 5% CO2, 37°C)
Primary HUVEC culture conditions (Endothelial Cell Growth Kit-BBE)

controls

Positive control: Primary HUVECs from ATCC
Negative control: Not explicitly mentioned

Annotations

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