Chitosan from crab shells (highly viscous, Product number: 48165, Lot # BCBP6349V) was purchased from Sigma-Aldrich (Saint Louis, MO, USA). The chitosan used was previously characterized and showed a molecular weight of 183 kDa [31 (link)]. Acetic acid (≥99.8%) was provided by Honeywell through Sigma-Aldrich (Saint Louis, MO, USA). Fetal bovine serum (FBS), DMEM low glucose, penicillin-streptomycin (P/S) and L-glutamine were obtained from Life Technologies (Gibco, Karlsruhe, Germany). Bacteriological agar was purchased from Scharlau (Ferrosa, Barcelona, Spain). Tryptic soy broth (TSB) and tryptic soy agar (TSA) were provided by Liofilchem (Roseto degli Abruzzi, Italy).
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