To examine the location of insulin-like growth factor II (IGF2) in human testicular tissues, we performed immunohistochemistry staining to detect the IGF2 expression. Tissues were cut into sections for immunoperoxidase staining after being treated with 4% PFA and paraffin wax. After the specific treatment with standard-procedure immunohistochemistry staining as described as Lian et al. [10 (link)], sections were incubated with IGF2 antibody (Abcam) overnight at 4°C and biotinylated secondary antibody (Abcam) for 2 h at room temperature.
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Immunohistochemical Localization of IGF2 in Human Testis
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Other organizations : First Affiliated Hospital of Anhui Medical University, Anhui Medical University
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- Treatment of tissue sections with 4% PFA and paraffin wax
- Incubation of sections with IGF2 antibody (Abcam) overnight at 4°C
- Incubation of sections with biotinylated secondary antibody (Abcam) for 2 h at room temperature
- Expression of IGF2 in human testicular tissues
- Use of standard-procedure immunohistochemistry staining as described in Lian et al. [10]
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
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