Annexin V and PI Staining for Apoptosis

Detection of apoptotic cells was done with fluorescein isothiocyanate- or allophycocyanin-labeled annexin V (BD Biosciences, Franklin Lakes, NJ, USA) and PI (Hoffman-La Roche, Basel, Switzerland), as described [53 (link)]. Apoptotic cells were analyzed on a BD FACSCalibur flow cytometer (BD Biosciences). Early apoptotic cells were stained with annexin V but not PI, whereas late apoptotic or necrotic cells were stained with both annexin V and PI.

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Suzuki R., Kitamura Y., Nakamura Y., Akashi H., Ogawa Y., Kawada H, & Ando K. (2020). Anti-tumor activities of the new oral pan-RAF inhibitor, TAK-580, used as monotherapy or in combination with novel agents in multiple myeloma. Oncotarget, 11(44), 3984-3997.

Publication 2020

fluorescein isothiocyanate- or allophycocyanin-labeled annexin V BD FACSCalibur flow cytometer

Allophycocyanin Annexin v Apoptotic Cells Fluorescein Isothiocyanate Necrotic

Corresponding Organization :

Other organizations : Tokai University

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Variable analysis

independent variables

Detection of apoptotic cells was done with fluorescein isothiocyanate- or allophycocyanin-labeled annexin V

dependent variables

Apoptotic cells were analyzed on a BD FACSCalibur flow cytometer
Early apoptotic cells were stained with annexin V but not PI
Late apoptotic or necrotic cells were stained with both annexin V and PI

control variables

PI (Hoffman-La Roche, Basel, Switzerland)

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