Cell culture, luciferase reporter assays, quantitative reverse transcriptase–polymerase chain reaction, Northern blot analyses, Western blot analyses, and immunochemistry were performed according to routine protocols. Generation of miR-22 conventional and cardiac-specific knockout (KO) mice, isoproterenol (ISO) administration, and measurement of cardiac function by echocardiography are described in the Online Materials and Methods .
Protocol detail
Comprehensive Cardiac Molecular Analyses
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Assays
Cardiac
Cell culture
Echocardiography
Function cardiac measurement
Isoproterenol
Knockout mice
Luciferase
Northern blot analyses
Reverse transcriptase polymerase chain reaction
Western blot analyses
Corresponding Organization :
Other organizations : Harvard Stem Cell Institute, Boston Children's Hospital, Harvard University, Chongqing Medical University
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Protocol cited in 19 other protocols
Variable analysis
independent variables
- Generation of miR-22 conventional and cardiac-specific knockout (KO) mice
- Isoproterenol (ISO) administration
- Measurement of cardiac function by echocardiography
- Cell culture
- Luciferase reporter assays
- Quantitative reverse transcriptase–polymerase chain reaction
- Northern blot analyses
- Western blot analyses
- Immunochemistry
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