Xenograft Tumor Model in Nude Mice

Five- to six-week-old male nude mice were purchase from Taconic (#NCRNU) for xenograft studies. A total of 1 x 10⁶ cells were re-suspended in 100 µl PBS solution and injected subcutaneously into the mice (n = 9 or 10 mice for each group) as described previously^{79 (link)}. At the end of experiment, mice were sacrificed and tumors were dissected for imaging and weighing. All mouse experiments were approved by the Institutional Animal Care and Use Committee (IACUC, RN150D) at Beth Israel Deaconess Medical Center (BIDMC). The Institute is committed to the highest ethical standards of care for animals used for the purpose of continued progress in the field of human cancer research. All mice were housed in a pathogen-free environment at BIDMC animal facility and were handled in strict accordance with the “Guide for the Care and Use of Laboratory Animals” and the applicable institutional regulations.