Sodium borohydride, sodium chloride, Dowex cation-exchange resin (50W-X8), ammonium bicarbonate (ABC), TFA, Dulbecco’s PBS (DPBS), hydrochloric acid (HCl), and dl-DTT were purchased from Sigma–Aldrich. Ethanol (Reag. Ph. Eur) and bovine submaxillary mucin (BSM), type I-S, were purchased from Merck. Tandem mass tag (TMT)pro label reagents, 8 M guanidine hydrochloride, Dulbecco’s modified Eagle’s medium, 0.25% trypsin/EDTA, and fetal calf serum (FCS) were obtained from Thermo Fisher Scientific. Potassium hydroxide was obtained from Honeywell Fluka. Solid phase extraction bulk sorbent carbograph was obtained from Grace Discovery Sciences. HPLC SupraGradient acetonitrile (methyl cyanide [MeCN]) was obtained from Biosolve. Peptide N-glycosidase F (PNGase F) and complete EDTA-free protease inhibitor cocktail tablets were purchased from Roche Diagnostics. A 96-well PP filter plate was purchased from Orochem Technologies. MultiScreen HTS 96 multiwell plates (hydrophobic Immobilon-P polyvinylidene difluoride [PVDF] membrane) and 96-well PP microplate were obtained from Millipore.
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