Western Blot Analysis of Cell Signaling

Samples were prepared as described previously^{40 (link)}. Anti AKT (#4691), pAKT (Ser473) (#4060), BAD (#9239), pBAD (Ser136) (#4366), BCL2 (#4223), BCL2A1 (#14093), BCL-W (#2724), BCLXL (#2764), pGSK3B (Ser9) (#9323), MCL1 (#39224), pMCL1 (Ser159/Thr163) (#4579), S6 (#2317) and pS6 (Ser235/236) (#2211) antibodies were purchased from Cell Signaling (New England Biolabs, Frankfurt, Germany). Anti GAPDH (ab8245) antibody was from Abcam (Cambridge, UK). Specific bands on nitrocellulose membranes were visualized with the biotin/streptavidin–horseradish peroxidase system (GE Healthcare, Little Chalfont, UK) in combination with the “Renaissance Western Blot Chemoluminescence Reagent” protocol (Perkin Elmer, Waltham, MA, USA).

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Quentmeier H., Geffers R., Hauer V., Nagel S., Pommerenke C., Uphoff C.C., Zaborski M, & Drexler H.G. (2022). Inhibition of MCL1 induces apoptosis in anaplastic large cell lymphoma and in primary effusion lymphoma. Scientific Reports, 12, 1085.

Publication 2022

pS6 (Ser235/236) biotin/streptavidin–horseradish peroxidase system Renaissance Western Blot Chemoluminescence Reagent

Antibodies Antibody Bcl2 Biotin Gapdh Horseradish peroxidase Mcl1 Membranes Nitrocellulose Streptavidin Western blot

Corresponding Organization : Leibniz Institute DSMZ – German Collection of Microorganisms and Cell Cultures

Other organizations : Helmholtz Centre for Infection Research

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Protein levels of AKT, pAKT (Ser473), BAD, pBAD (Ser136), BCL2, BCL2A1, BCL-W, BCLXL, pGSK3B (Ser9), MCL1, pMCL1 (Ser159/Thr163), S6, and pS6 (Ser235/236)

control variables

None explicitly mentioned

controls

Positive control: None mentioned
Negative control: None mentioned

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