Synthesis and Characterization of Cyclic-di-GMP Analogs

Cyclic-di-GMP and its analogs were chemically synthesized as described previously (Hayakawa et al., 2003 ; Hyodo et al., 2006 ;). Cyclic-di-GMP analogs used in this study were cyclic bis(3′–5′)guanylic/adenylic acid (cyclic-GpAp or c-GpAp), cyclic bis(3′–5′)guanylic/inosinic acid (cyclic-GpIp or c-GpIp) and monophosphorothioic acid of cyclic-di-GMP (cyclic-GpGps or c-GpGps) (Hyodo et al., 2006 ). The structures of these compounds are shown in Fig. 1. These compounds were dissolved in 0.85% NaCl for use.

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Ishihara Y., Hyodo M., Hayakawa Y., Kamegaya T., Yamada K., Okamoto A., Hasegawa T, & Ohta M. (2009). Effect of cyclic bis(3′–5′)diguanylic acid and its analogs on bacterial biofilm formation. Fems Microbiology Letters, 301(2), 193-200.

Publication 2009

Acid Cyclic di gmp Guanylic acid Inosinic acid Nacl

Corresponding Organization : Nagoya University

Other organizations : Nagoya City University

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Variable analysis

independent variables

Cyclic-di-GMP and its analogs: cyclic bis(3′–5′)guanylic/adenylic acid (cyclic-GpAp or c-GpAp), cyclic bis(3′–5′)guanylic/inosinic acid (cyclic-GpIp or c-GpIp), and monophosphorothioic acid of cyclic-di-GMP (cyclic-GpGps or c-GpGps)

dependent variables

Not explicitly mentioned

control variables

The compounds were dissolved in 0.85% NaCl for use.

controls

No positive or negative controls were explicitly mentioned.

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