Sanger Sequencing for Pathogenic Variant Confirmation

Possible pathogenic variants were confirmed by performing Sanger sequencing with an ABI 3500XL DX Genetic Analyzer (Applied Biosystems, Foster City, CA, USA) following the procedure described in our previous study.20 (link) A co-segregation analysis was performed for all available familial members. The APOE genotypes were determined by multiplex amplification refractory mutation system PCR according as previously described.20 (link)

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Han L.H., Xue Y.Y., Zheng Y.C., Li X.Y., Lin R.R., Wu Z.Y, & Tao Q.Q. (2020). Genetic Analysis of Chinese Patients with Early-Onset Dementia Using Next-Generation Sequencing. Clinical Interventions in Aging, 15, 1831-1839.

Publication 2020

ABI 3500XL DX Genetic Analyzer

Apoe Familial members Genetic Genotypes Multiplex pcr Mutation Pathogenic

Corresponding Organization :

Other organizations : Second Affiliated Hospital of Zhejiang University, Taizhou Second People's Hospital, Tulane University

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Presence of pathogenic variants
APOE genotypes

control variables

None explicitly mentioned

controls

Co-segregation analysis was performed for all available familial members.

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