Niacin Modulates Neutrophil Respiratory Burst
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Corresponding Organization : University of Cincinnati Medical Center
Other organizations : Max Planck Institute for Heart and Lung Research
Variable analysis
- Niacin (50 µM or 300 µM)
- Phorbol 12-myristate 13-acetate (PMA, 100 nM)
- Luminescence
- Human promyelocytic NB4 cells differentiated into neutrophilic cells by treating with all-trans retinoic acid (ATRA, 1 µM) for 5 days
- Cells (5 × 10^4/well in 96 well plates) incubated in phenol-free medium containing L-012 for 10 min
- Positive control: Cells treated with PMA (100 nM)
- Negative control: Cells not treated with PMA (100 nM)
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