Overexpression of SNORA42 in HEK293T

SNORA42 was cloned as described previously.^{21 (link)} A pCDH vector (System biosciences, Mountain view, CA, USA) was used for ectopic over-expression of SNORA42. The pCDH vector encoding intact sequence of SNORA42 cDNA or empty vector as a control was infected into HEK293T cells together with pPACKH1 Packaging Plasmid mix (System biosciences) for producing viral particles using Lipofectamine 2000 (Invitrogen, Carlsbad, CA). Further information is provided in the Supplementary material and methods.

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Okugawa Y., Toiyama Y., Toden S., Mitoma H., Nagasaka T., Tanaka K., Inoue Y., Kusunoki M., Boland C.R, & Goel A. (2015). Clinical significance of SNORA42 as an oncogene and a prognostic biomarker in colorectal cancer. Gut, 66(1), 107-117.

Publication 2015

pCDH vector pPACKH1 Packaging Plasmid mix Lipofectamine 2000

Cdna Cells Ectopic expression Lipofectamine 2000 Plasmid Vector Viral particles

Corresponding Organization :

Other organizations : Center for Cancer Research, Baylor University Medical Center, Mie University, Institute of Life Sciences, Baylor University, Baylor Scott & White Health, Okayama University

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Variable analysis

independent variables

Ectopic over-expression of SNORA42

dependent variables

Not explicitly mentioned

control variables

Empty vector as a control

controls

Negative control: Empty vector

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