Chromatographic Profiling of Polyphenols
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Corresponding Organization : University of Manitoba
Protocol cited in 3 other protocols
Variable analysis
- Elution gradient (0-3 min 99% A, 1% B; 30 min 5% A, 95% B; 40 min 99% A, 1% B)
- Chromatographic profiles of P90, E90, E80, and E70 samples
- Polyphenols content calculated with standard molecules of phloroglucinol and resorcinol
- HPLC system (Jasco, Tokyo, Japan) equipped with a PU-980 pump, an UV-1575 detector, and a degasser Populaire DP4003
- PowerChrom 2.5 (eDaq Technologies, NZ, Australia) software and MATLAB R2019b software (MathWorks Inc., USA) for data acquisition and processing
- Kromasil C-18 semi-preparative column (8 × 250 mm) at 30°C
- Freeze-dried samples (2 mg/ml) diluted in EtOH 20% (v/v) and filtered through 0.45 µm syringe filters
- Injection volume of 50 µl, detection wavelength of 266 nm, and solvent flow rate of 0.4 ml/min
- Glucose and glucuronic acid standards injected to check for possible interference of UA and sugars
- Glucose and glucuronic acid standards were injected to check for possible interference of UA and sugars
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