For determination of RNA half-lives in biofluids, 1 μg heated and refolded U2-OS total RNA was incubated at 37 °C for variable periods with 50 μL undiluted or PBS-diluted biofluid samples. Digested RNAs were purified by SPE and analyzed by northern blot.
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Determining RNA Half-Lives in Biofluids
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Corresponding Organization :
Other organizations : Universidad de la República, Institut Pasteur de Montevideo, University of California, San Diego, Brigham and Women's Hospital, Harvard University, Precision for Medicine (United States), Johns Hopkins University, Hospital de Clínicas
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Variable analysis
independent variables
- Incubation time (variable periods)
- RNA half-lives in biofluids
- 1 μg heated and refolded U2-OS total RNA
- 37 °C incubation temperature
- 50 μL undiluted or PBS-diluted biofluid samples
- Positive control: Not explicitly mentioned.
- Negative control: Not explicitly mentioned.
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