Cultivation of Methanogenic Archaea Strains

M. smithii ATCC 35061^T DSMZ 861, M. smithii DSMZ 2374, M. smithii DSMZ 2375, M. smithii DSMZ 11975, M. oralis DSMZ 7256 ^T, M. stadtmanae ATCC 43021^T DSMZ 3091, M. beijingense DSMZ 15999 and M. concilii DSMZ 2139 purchased from the German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig, Germany). The M. arboriphilicus strain tested in this study was recently isolated in our laboratory from one human stool specimen. The M. smithii strains, M. arboriphilicus and M. beijingense[6] were grown in liquid medium 119 (http://www.dsmz.de). To cultivate M. oralis, medium 119 was modified by the addition of 1 g/L of yeast extract and 1 g/L of peptone, and a 2.5 bar H₂/CO₂ (80%-20%) atmosphere was used. Medium 322 (http://www.dsmz.de) was used to cultivate M. stadtmanae. Medium 334c was used (http://www.dsmz.de) to cultivate M. concilii at 37°C in Hungate tubes (Dutscher, Issy-les-Moulineaux, France) under a 2-bar H₂/CO₂ (80%–20%) atmosphere with agitation. M. luminyensis CSUR P135^T was cultivated using the Methanobrevibacter medium (medium 119: http://www.dsmz.de) modified by the addition of methanol and selenite/tungstate solution under 2-bar of H₂/CO₂ (80%-20%) atmosphere with agitation [3] .