Apoptosis Detection Protocols

An Annexin V-PI apoptosis detection kit (Vazyme Biotech Co. Ltd., China) was used in a manner similar to that of a previous description [22 (link), 23 (link)] to detect apoptosis. Thereafter, fluorescence-activated cell sorting (FACS) was utilized to count the stained cells using CellQuest software (BD Biosciences, USA) connected to a Calibur flow cytometer. A TUNEL FITC Apoptosis Detection Kit (Vazyme Biotech Co. Ltd., China) was used, as instructed by the manufacturer, to conduct TUNEL staining. Immunofluorescence was observed using a Nikon ECLIPSE E800 fluorescence microscope.

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Wei S., Peng L., Yang J., Sang H., Jin D., Li X., Chen M., Zhang W., Dang Y, & Zhang G. (2020). Exosomal transfer of miR-15b-3p enhances tumorigenesis and malignant transformation through the DYNLT1/Caspase-3/Caspase-9 signaling pathway in gastric cancer. Journal of Experimental & Clinical Cancer Research : CR, 39, 32.

Publication 2020

Annexin V-PI apoptosis detection kit CellQuest software Calibur flow cytometer TUNEL FITC Apoptosis Detection Kit ECLIPSE E800 fluorescence microscope

Annexin v Apoptosis Cells Fitc Fluorescence microscope Immunofluorescence Tunel

Corresponding Organization :

Other organizations : Nanjing Medical University, Jiangsu Province Hospital

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Variable analysis

independent variables

Apoptosis detection methods: Annexin V-PI and TUNEL staining

dependent variables

Apoptosis levels measured by fluorescence-activated cell sorting (FACS) and immunofluorescence microscopy

control variables

Not explicitly mentioned

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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