Measuring Wnt/β-Catenin Signalling Activity
Corresponding Organization :
Other organizations : Second Affiliated Hospital of Zhejiang University, Kagawa University, Kobe University, Fourth Hospital of Hebei Medical University, Hebei Medical University
Variable analysis
- SiRNA transfection
- PENTR4 plasmid vector transfection
- Wnt/β-catenin signalling activity measured using a β-catenin firefly luciferase reporter plasmid containing TCF/LEF binding sites (TOP flash)
- Luminescence intensity
- PRL-TK renilla luciferase reporter vector for transfection efficiency control
- β-catenin reporter plasmid containing mutated TCF/LEF binding sites (FOP flash) as negative control
Annotations
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