In the first four experiments, eight-month-old Q. affinis plants were used as rootstock and in the fifth experiment, Q. rugosa and Q. affinis rootstocks (Table 1).
The seed used to produce Q. affinis rootstocks was obtained from the GUMAIR nursery in the region of Acaxochitlán, Hidalgo. Transplanting was done after six months in 4 L bags with a substrate based on 60 % of oak bark and 40 % of composted pine bark. At the time of grafting, the rootstocks measured 50 cm in height and 1.5 cm in average diameter. The Q. rugosa rootstocks were two years old, 50 cm in height and 2 cm in average diameter and were in 2 L black polyethylene bags with a substrate mixture of oak bark and compost in a 3:1 ratio. The plant was produced at the Finca M Y M SPR de RL nursery in Zacatlán, Puebla, located at 19° 59' 48.43" N and 97° 59' 26. 56" W.
Yara® (DAP) 18-46-00 (N-P-K) slow-release fertilizer plus micronutrients (1 g•L -1 ) and Trichoderma harzianum strain ISF13 (2.5 x 10 4 conidia•mL -1 ) dissolved in water were applied to each rootstock. Subsequently, lateral branches were removed from the rootstocks and the apical shoot was left. Quaternary ammonium salts were applied as a disinfectant in the area where the cutting was performed.
injertos se hicieron con varetas herbáceas, semileñosas y leñosas de fenotipos tolerantes y susceptibles. La cantidad de injertos por árbol donante varió debido a que muchas púas eran delgadas y de yemas pequeñas; es decir, sin vigor y, además, lignificadas. Las varetas seleccionadas tenían vigor alto, consistencia semileñosa, entrenudos cortos y yemas grandes en el final de su latencia. En los árboles tolerantes y susceptibles se realizaron la misma cantidad de injertos.
Tres experimentos se realizaron en 2020 (verano: 8 de septiembre, 8 de octubre y 10 de noviembre) y dos en 2021 (invierno [4 de marzo] y otoño [28 de septiembre]).
The seed used to produce Q. affinis rootstocks was obtained from the GUMAIR nursery in the region of Acaxochitlán, Hidalgo. Transplanting was done after six months in 4 L bags with a substrate based on 60 % of oak bark and 40 % of composted pine bark. At the time of grafting, the rootstocks measured 50 cm in height and 1.5 cm in average diameter. The Q. rugosa rootstocks were two years old, 50 cm in height and 2 cm in average diameter and were in 2 L black polyethylene bags with a substrate mixture of oak bark and compost in a 3:1 ratio. The plant was produced at the Finca M Y M SPR de RL nursery in Zacatlán, Puebla, located at 19° 59' 48.43" N and 97° 59' 26. 56" W.
Yara® (DAP) 18-46-00 (N-P-K) slow-release fertilizer plus micronutrients (1 g•L -1 ) and Trichoderma harzianum strain ISF13 (2.5 x 10 4 conidia•mL -1 ) dissolved in water were applied to each rootstock. Subsequently, lateral branches were removed from the rootstocks and the apical shoot was left. Quaternary ammonium salts were applied as a disinfectant in the area where the cutting was performed.
injertos se hicieron con varetas herbáceas, semileñosas y leñosas de fenotipos tolerantes y susceptibles. La cantidad de injertos por árbol donante varió debido a que muchas púas eran delgadas y de yemas pequeñas; es decir, sin vigor y, además, lignificadas. Las varetas seleccionadas tenían vigor alto, consistencia semileñosa, entrenudos cortos y yemas grandes en el final de su latencia. En los árboles tolerantes y susceptibles se realizaron la misma cantidad de injertos.
Tres experimentos se realizaron en 2020 (verano: 8 de septiembre, 8 de octubre y 10 de noviembre) y dos en 2021 (invierno [4 de marzo] y otoño [28 de septiembre]).
