HCVpp and VSVpp were produced as described previously [28 (link),29 (link)] with plasmids kindly provided by B. Bartosch and F.L. Cosset (Lyon, France). Pseudotyped particles were inoculated on 293T, 786-O or Huh-7 cells for 3 h at 37°C. At 72 h post-infection, cells were lysed and processed to measure the Firefly luciferase activities as indicated by the manufacturer (Promega). Luciferase activities with HCVpp were normalized for luciferase activities obtained with VSVpp. In each figure, results are reported as the mean ± S.D. of at least three independent experiments.
Fénéant L., Ghosn J., Fouquet B., Helle F., Belouzard S., Vausselin T., Séron K., Delfraissy J.F., Dubuisson J., Misrahi M, & Cocquerel L. (2015). Claudin-6 and Occludin Natural Variants Found in a Patient Highly Exposed but Not Infected with Hepatitis C Virus (HCV) Do Not Confer HCV Resistance In Vitro. PLoS ONE, 10(11), e0142539.
Other organizations :
Center for Infection and Immunity of Lille, Inserm, CNR de la Résistance aux Antibiotiques, Hôtel-Dieu de Paris, Centre Hospitalier Universitaire Amiens-Picardie, Assistance Publique – Hôpitaux de Paris, Université Paris-Sud, Université Paris-Saclay
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