Profiling Immune Cell Responses to Tumor Antigen

Rabbit anti-human Carcinoembryonic Antigen polyclonal antibody (Ab) (Dako, Denmark Ab A0115); rabbit IgG control (Vector, CA, USA); PE-conjugated anti-CEACAM1 monoclonal antibody (mAb) (R and D Systems MAb2244); FITC-conjugated mouse anti-human CD14 mAb, human FcR Blocking Reagent, CD14+/CD15+ Microbeads and LS columns (Miltenyi biotec, Germany); PE-conjugated mouse anti-human CD86 mAb and PE-Cy7-conjugated mouse anti-human CD80 mAb (Biolegend, CA, USA); PE-conjugated mouse anti-human CD206 mAb and FITC-conjugated mouse anti-human CD15 mAb (Becton Dickinson, CA, USA); PE-conjugated goat anti-rabbit IgG antibody, endotoxin-free water and Histopaque-1077 (Sigma, USA); fixable Vital dye eFluor 780 (ebioscience, CA, USA); LPS-EK Ultrapure (Invivogen, CA, USA); Ni-NTA agarose and polypropylene column (QIAGEN); Pierce BCA Protein Assay Kit and Pierce LAL Chromogenic Endotoxin Quantitation Kit (Thermo Scientific); Proteome Profiler kits (R&D systems, UK). Antiserum to recombinant rD-7 was raised in mice and has been described previously [29] (link).