Ischemic Penumbra Protein Analysis

The ischemic penumbra of rat brain tissues (n = 6) was collected for Western blot analysis as previously described (Liu et al., 2020 (link)). The following primary antibodies were used: anti‐beta‐Actin (Abcam, ab8226, 1:1000), anti‐GPX4 (Abcam, ab252833, 1:1000), anti‐FTH1 (Abcam, ab183781, 1:1000), anti‐TF (Abcam, ab82411, 1:1000), anti‐TF receptor (Abcam, ab269513, 1:1000), anti‐HO1 (Abcam, ab68477, 1:1000), anti‐ACSL‐4 (Abcam, ab155282, 1:1000), and anti‐xCT (Invitrogen, PA1‐16893, 1:1000).

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Sun M., Liu M., Li Q., Zhang X., Liu S., Yang H., Yang L., Tian J., Mi W, & Ma Y. (2023). Cottonseed oil alleviates ischemic stroke injury by inhibiting ferroptosis. Brain and Behavior, 13(10), e3179.

Publication 2023

ab8226 ab252833 ab183781 ab82411 ab269513 ab68477 PA1‐16893

Antibodies Beta actin Brain Gpx4 Tissues Western blot analysis

Corresponding Organization : Chinese PLA General Hospital

Other organizations : Jinzhou Medical University, Beijing Tongren Hospital, Capital Medical University, Affiliated Hospital of Nantong University, Nantong University

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Ischemic penumbra of rat brain tissues (n = 6)

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