The qRT-PCR assay was performed according to the method described previously (Zhu et al., 2020 (link)). The 16S RNA was used as the internal reference gene (Zhu et al., 2020 (link)). All RT-PCRs were performed using CFX96 Real-Time PCR Detection System (Bio-Rad, Hercules, USA). In this study, all tests were performed in triplicate. The data were analyzed using SPSS statistical analysis software version 17.0 (SPSS Inc., Armonk, NY, USA).
Free full text: Click here