Total RNA was isolated as previously described24 (link). Briefly, with the purpose of degrading the residual RNA outside the vesicles, the exosome suspension was treated with RNAse A (Qiagen NV; Germany) (100 μg/ml final reaction concentration; 15 min at 37 °C). Total RNA was obtained from exosomes using the miRCURY RNA Isolation Kit-Cell and Plant (Exiqon; Denmark). RNA concentration was calculated by using the QUBIT fluorometer and the Quant-iT RNA Assay kit (Invitrogen; California, USA). All RNA samples presented an OD 260/280 nm ratio ≥ 1,7 when using a Nanodrop UV-Vis spectrophotometer (Thermo Fisher Scientific; Massachusetts, USA).
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