Chronic Electrode Implantation for VTA Stimulation

Monkeys were fitted with MRI-compatible chambers (Crist Instruments) and a chronic electrode array was implanted using peri-operative MRI guidance^{19 (link)}. The micro-brush electrode arrays^{66 (link)} used to stimulate VTA consisted of 34 Pt/Ir microwires with polyimide insulation threaded through a 26 G microfil tube. The microwires of each electrode array consisted of a mix of 25 and 50 μm diameter wires. The microwires were attached to a 36-pin omnetics connector and were uniformly cut 5 mm past the microfil tube at the recording end. Unipolar electrical microstimulation was performed using the stimulating electrodes in the VTA and a low impedance ground wire implanted below the skull. The electrical microstimulation signal was produced with a stimulus isolator (DLS100, World Precision Instruments, current mode) connected to an eight-channel digital stimulator (DS8000, World Precision Instruments) and triggered by custom software that also controlled the visual and behavioral paradigms. Electrical microstimulation events were composed of stimulation trains lasting 200 ms and were composed of biphasic, square-wave pulses with a repetition rate of 100 Hz. Each pulse consisted of 0.2 ms positive and 0.2 ms negative voltage performed at 5 ms intervals. These stimulation parameters were used in experiments 1 and 2.

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Arsenault J.T, & Vanduffel W. (2019). Ventral midbrain stimulation induces perceptual learning and cortical plasticity in primates. Nature Communications, 10, 3591.

Publication 2019

DLS100 DS8000

Digital Electrical Microfil Monkeys Pulse Pulses Skull

Corresponding Organization :

Other organizations : KU Leuven

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Variable analysis

independent variables

Electrical microstimulation of the ventral tegmental area (VTA)

dependent variables

Measured outcomes not explicitly mentioned

control variables

Unipolar electrical microstimulation using a low impedance ground wire implanted below the skull
Stimulus isolator (DLS100, World Precision Instruments, current mode) connected to an eight-channel digital stimulator (DS8000, World Precision Instruments)
Electrical microstimulation events composed of stimulation trains lasting 200 ms, biphasic, square-wave pulses with a repetition rate of 100 Hz, and each pulse consisting of 0.2 ms positive and 0.2 ms negative voltage performed at 5 ms intervals

controls

No positive or negative controls explicitly mentioned

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