Lentiviral knockdown of circHIPK3 and HIPK3

CircHIPK3, small interfering RNAs (siRNAs) against circHIPK3, the linear HIPK3 gene, and siRNAs against HIKP3 were transfected into CMs or cardiac endothelial cells using a lentiviral construct according to the manufacturer's protocol (Hanbio, China) and performed as previously published [11 (link)]. In addition, miR-29a mimic, inhibitor, or their corresponding negative controls (RiboBio, China) were transfected into cardiac endothelial cells with Lipofectamine 3000 (Invitrogen, USA) according to the kit's instructions. At 48 h after transfection, qPCR was used to evaluate the gene expression in cardiac endothelial cells.