Molecular Targets of Small-Molecule Inhibitors

Phenyl vinyl sulfonate and phenyl vinyl sulfone (PVS) were purchased from Enamine Ltd (Monmouth, NJ). JTT-551 (PTP1B and TCPTP inhibitor; IC50 = 0.2 and 2 µM, respectively), NSC-87877 (SHP-1 and SHP-2 inhibitor; IC50 = 0.36 and 0.32 µM, respectively), and CX08005 (PTP1B and TCPTP inhibitor; IC50 = 0.78 and 0.48 µM, respectively) were obtained from Millipore Sigma (Darmstadt, Germany). β-actin mouse monoclonal antibody (clone AC-74, catalog number A5316) was obtained from Sigma-Aldrich (St. Louis, MO). STAT1 (rabbit monoclonal, catalog number 9175), phospho-STAT1 (tyrosine 701, rabbit monoclonal, catalog number 9167), phospho-STAT3 (tyrosine 705, rabbit monoclonal, catalog number 9131), Caspase-3 (rabbit monoclonal, catalog number 14220), anti-biotin HRP linked (goat, catalog number 7075), anti-rabbit (polyclonal) and anti-mouse (polyclonal) antibodies and cytokines were purchased from Cell Signaling Technology (Danvers, MA). Sodium vanadate was dissolved in water, sterile filtered and stored at 4 °C until use (Hill III & Rice, 2018 (link)).

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Hong B.V., Lee J.H, & Rice R.H. (2020). Off-target effects of protein tyrosine phosphatase inhibitors on oncostatin M-treated human epidermal keratinocytes: the phosphatase targeting STAT1 remains unknown. PeerJ, 8, e9504.

Publication 2020

NSC-87877 phospho-STAT1 phospho-STAT3 Caspase-3 anti-biotin HRP linked

Actin Antibodies Biotin Caspase 3 Clone Cx08005 Cytokines Goat Jtt 551 Monoclonal antibody Mouse Nsc 87877 Phenyl vinyl sulfone Ptp1b Rabbit Rice Shp 2 Sodium vanadate Stat1 Stat3 Sterile Sulfonate Tcptp Tyrosine Vinyl

Corresponding Organization :

Other organizations : University of California, Davis

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Variable analysis

independent variables

Phenyl vinyl sulfonate
Phenyl vinyl sulfone (PVS)
JTT-551 (PTP1B and TCPTP inhibitor; IC50 = 0.2 and 2 µM, respectively)
NSC-87877 (SHP-1 and SHP-2 inhibitor; IC50 = 0.36 and 0.32 µM, respectively)
CX08005 (PTP1B and TCPTP inhibitor; IC50 = 0.78 and 0.48 µM, respectively)

dependent variables

STAT1 phosphorylation (tyrosine 701)
STAT3 phosphorylation (tyrosine 705)
Caspase-3 activation

control variables

β-actin (used as a loading control)
Sodium vanadate (dissolved in water, sterile filtered and stored at 4 °C until use)

positive controls

None mentioned

negative controls

None mentioned

Annotations

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