MCF Derivatization and GC-MS Analysis

All prepared extracts were chemically modified to lower their boiling point by MCF derivatization, based on the method published in Nature protocols (36 (link)). The volatile compounds were then separated by ZB-1701 GC capillary column (30 m × 250 μm id × 0.15 μm with 5 m guard column, Phenomenex, CA, USA) and detected by GC-MS (Agilent 7890B-5977A) with electron impact ionization via electron emission at 70 eV. The GC-MS parameters were operated following the procedure in previous research (37 (link)). The GC-MS inlet was set at 290°C with the pulsed splitless mode, 1 ml/min in the flow rate of the helium carrier. The temperature was controlled at 280°C, 230°C, and 150°C of auxiliary, MS quadrupole, and MS source respectively. The mass range was detected between 30 μm to 550 μm, with a scan speed of 1.562 μ/s and the mass spectrometry detector turned on after 5.5 min.

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Zhong X., Ran R., Gao S., Shi M., Shi X., Long F., Zhou Y., Yang Y., Tang X., Lin A., He W., Yu T, & Han T.L. (2022). Complex metabolic interactions between ovary, plasma, urine, and hair in ovarian cancer. Frontiers in Oncology, 12, 916375.

Publication 2022

ZB-1701 GC capillary column 5 m guard column

Capillary Electron Gc ms Helium Mass spectrometry Scan

Corresponding Organization :

Other organizations : Dalian Medical University, Chongqing Medical University, Second Affiliated Hospital of Chongqing Medical University, First Affiliated Hospital of Chongqing Medical University, University of Auckland

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Variable analysis

independent variables

MCF derivatization method to lower boiling point of prepared extracts

dependent variables

Volatile compound separation by ZB-1701 GC capillary column
Volatile compound detection by GC-MS with electron impact ionization

control variables

GC-MS parameters (inlet temperature, flow rate, auxiliary temperature, MS quadrupole temperature, MS source temperature, mass range, scan speed)
GC-MS instrument (Agilent 7890B-5977A)

controls

No positive or negative controls were explicitly mentioned in the provided information.

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