Silencing DNMT1 and HDAC1 in SHR cells

Validated siRNA for DNMT1 (ID: S4215) and HDAC1 (ID: S73) and the positive/negative control RNAi (Thermo Scientific, Applied Biosystems) were transfected into early transformed SHR cells by indicated treatment using the Silencer siRNA Transfection II Kit (Thermo Scientific) according to the protocols provided by the manufacturer [23 (link)].

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Li Y., Buckhaults P., Cui X, & Tollefsbol T.O. (2016). Combinatorial epigenetic mechanisms and efficacy of early breast cancer inhibition by nutritive botanicals. Epigenomics, 8(8), 1019-1037.

Publication 2016

Silencer siRNA Transfection II Kit

Cells Dnmt1 Rnai Sirna Transfection

Corresponding Organization :

Other organizations : University of Alabama at Birmingham, University of South Carolina

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Variable analysis

independent variables

Validated siRNA for DNMT1 (ID: S4215)
Validated siRNA for HDAC1 (ID: S73)

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

controls

Positive control RNAi
Negative control RNAi

Annotations

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