Protein Expression Analysis by Western Blotting

Western blotting was performed as previously described [32 (link)].The antibodies used were as follows: anti-FGF19 (A6589, ABclonal, China, 1:500), anti-TRIM21 (12108–1-AP, Proteintech, China, 1:1000), anti-ANXA2 (sc-28385, Santa Cruz Biotechnology, Texas, USA, 1:1000), anti-Tubulin(FD0064, Fude bio, China, 1:1000), anti-p-PI3K (A0982, ABclonal, China, 1:500), anti-AKT1 (A11016, ABclonal, China, 1:1000), anti-p-AKT1 (AP0140, ABclonal, China, 1:1000), anti-mTOR (A11355, ABclonal, China, 1:500), and anti-p-mTOR (ab109268, Abcam, UK, 1:1000).

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Shi S., Zhang Q., Zhang K., Chen W., Xie H., Pan S., Xue Z., You B., Zhao J, & You Y. (2023). FGF19 promotes nasopharyngeal carcinoma progression by inducing angiogenesis via inhibiting TRIM21-mediated ANXA2 ubiquitination. Cellular Oncology (Dordrecht), 47(1), 283-301.

Publication 2023

anti-ANXA2 sc-28385 A11016 AP0140 A11355 ab109268

Corresponding Organization : Affiliated Hospital of Nantong University

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Variable analysis

independent variables

Anti-FGF19 (A6589, ABclonal, China, 1:500)
Anti-TRIM21 (12108–1-AP, Proteintech, China, 1:1000)
Anti-ANXA2 (sc-28385, Santa Cruz Biotechnology, Texas, USA, 1:1000)
Anti-Tubulin(FD0064, Fude bio, China, 1:1000)
Anti-p-PI3K (A0982, ABclonal, China, 1:500)
Anti-AKT1 (A11016, ABclonal, China, 1:1000)
Anti-p-AKT1 (AP0140, ABclonal, China, 1:1000)
Anti-mTOR (A11355, ABclonal, China, 1:500)
Anti-p-mTOR (ab109268, Abcam, UK, 1:1000)

dependent variables

Protein expression levels of FGF19, TRIM21, ANXA2, Tubulin, p-PI3K, AKT1, p-AKT1, mTOR, and p-mTOR

control variables

Western blotting was performed as previously described [32 (link)]

controls

No positive or negative controls were explicitly mentioned.

Annotations

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