Humoral Immune Response to Influenza

Anti-influenza IgG, IgG1, and IgG2c humoral responses were measured by enzyme-linked immunosorbent assay (ELISA) as previously described (4 (link)). Briefly, ELISA plates (Immulon) were coated with 100 ng of inactivated influenza virus. Serially diluted mouse serum samples were added to the wells, and bound immunoglobulins were detected with the appropriate secondary antibody (goat anti-mouse IgG [1:10,000, Sigma-Aldrich], rat anti-mouse IgG1 [1:2,000, SouthernBiotech], or goat anti-mouse IgG2c [1:4,000, SouthernBiotech]. A titer was designated as the serum dilution corresponding to the inflection point of the plot of the optical density vs. dilution of serum. Hemagglutination inhibition (HAI) titers in sera samples were determined by SRI International (Harrisburg, VA).

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Morse K., Kimizuka Y., Chan M.P., Shibata M., Shimaoka Y., Takeuchi S., Forbes B., Nirschl C., Li B., Zeng Y., Bronson R.T., Katagiri W., Shigeta A., Sîrbulescu R.F., Chen H., Tan R.Y., Tsukada K., Brauns T., Gelfand J., Sluder A., Locascio J.J., Poznansky M.C., Anandasabapathy N, & Kashiwagi S. (2017). Near-infrared 1064 nm laser modulates migratory dendritic cells to adjuvant the immune response to intradermal influenza vaccine. Journal of immunology (Baltimore, Md. : 1950), 199(4), 1319-1332.

Publication 2017

goat anti-mouse IgG rat anti-mouse IgG1 goat anti-mouse IgG2c

Anti igg Antibody Dilution Enzyme linked immunosorbent assay Goat Hemagglutination inhibition Igg1 Influenza Influenza virus Mouse Point Sera

Corresponding Organization : Massachusetts General Hospital

Other organizations : Brigham and Women's Hospital, Harvard University, Keio University

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Variable analysis

independent variables

Anti-influenza IgG, IgG1, and IgG2c humoral responses

dependent variables

Optical density vs. dilution of serum
Hemagglutination inhibition (HAI) titers in sera samples

control variables

Enzyme-linked immunosorbent assay (ELISA) plates coated with 100 ng of inactivated influenza virus
Serially diluted mouse serum samples

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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