Immunocytochemistry was carried out on fixed unpermeabilized cells with polyclonal antibodies anti-XlEnpp4 used at 1/200 or anti-mEnpp4 (CR65; see ref. 31 (link)) used at 1/400 and anti-rabbit IgG FITC (Sigma) at 1/80. The staining was recorded using a Nikon Optiphot/ Diginet camera system. Photographs were taken at a magnification of ×40.
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