Overexpression of Circ-ATXN2 in Rat ASCs

To overexpress circ-ATXN2, we used the pLC5-ciR-GFP (#GS0108) vector (Geneseed, Guangzhou, China). We inserted the full-length circ-ATXN2 (chr12: 40329454-40335680) into the pLC5-ciR-GFP vector between the restriction sites. Thereafter, the vector was packaged into the lentivirus and infected into rat ASCs (Sun et al., 2020 (link)). The vector-GFP- and circ-ATXN2–GFP-expressing cells were sorted for subsequent experiments using a Moflo Astrios^EQ flow cytometer (Beckman Coulter, United States).

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Song X.H., He N., Xing Y.T., Jin X.Q., Li Y.W., Liu S.S., Gao Z.Y., Guo C., Wang J.J., Huang Y.Y., Hu H, & Wang L.L. (2021). A Novel Age-Related Circular RNA Circ-ATXN2 Inhibits Proliferation, Promotes Cell Death and Adipogenesis in Rat Adipose Tissue-Derived Stromal Cells. Frontiers in Genetics, 12, 761926.

Publication 2021

Moflo AstriosEQ flow cytometer

Ascs Cells Lentivirus Vector

Corresponding Organization :

Other organizations : Zhejiang University, Sir Run Run Shaw Hospital, China Academy of Chinese Medical Sciences, Zhejiang Chinese Medical University

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Variable analysis

independent variables

Overexpression of circ-ATXN2 using the pLC5-ciR-GFP vector

dependent variables

Cells expressing vector-GFP and circ-ATXN2–GFP, which were sorted for subsequent experiments

control variables

Not explicitly mentioned

controls

No positive or negative controls were explicitly mentioned in the provided information

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