Purification of Primary Myeloid DCs

Primary circulating CD11c+ mDC were directly purified by negative immunomagnetic selection using the human CD1c (BDCA-1+) Dendritic cell Isolation Kit (miltenyi Biotec) from PBMC of HIV-1 EC and healthy donors. Monocyte-derived-DC (MDDC) were generated from HIV-1 negative circulating adherent monocytes and cultured during 6 days in the presence of recombinant GMCSF and IL-4 as previously described (34 (link)).

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Martin-Gayo E., Gao C., Calvet-Mirabent M., Ouyang Z., Lichterfeld M, & Yu X.G. (2022). Cooperation between cGAS and RIG-I sensing pathways enables improved innate recognition of HIV-1 by myeloid dendritic cells in elite controllers. Frontiers in Immunology, 13, 1017164.

Publication 2022

human CD1c (BDCA-1+) Dendritic cell Isolation Kit

Bdca 1 Cell isolation Dendritic Dendritic cell Donors Gmcsf Hiv 1 Human Isolation Monocytes

Corresponding Organization : Massachusetts General Hospital

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Variable analysis

independent variables

Purification of primary circulating CD11c+ mDC from PBMC of HIV-1 EC and healthy donors
Generation of Monocyte-derived-DC (MDDC) from HIV-1 negative circulating adherent monocytes and cultured during 6 days in the presence of recombinant GMCSF and IL-4

dependent variables

Phenotype and function of primary circulating CD11c+ mDC and MDDC

control variables

Healthy donors
HIV-1 negative circulating adherent monocytes

controls

Positive control: Primary circulating CD11c+ mDC from healthy donors
Negative control: HIV-1 negative circulating adherent monocytes

Annotations

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