Visualizing Coacervate Fusion Dynamics

Fusion events of coacervates were collected at a rate of 65 ms per frame using a wide-field Axio Observer 7 Inverted Microscope (Zeiss) with a ×63/1.4-NA Plan-Apochromat (oil immersion) objective. Cover glass bottoms were treated with 1% Pluronics F127 for 25 to 30 min and washed with deionized water prior to protein incubation. Images were acquired with an Axiocam 506 mono camera (Zeiss) controlled by the Zen software (Zeiss). ImageJ was used for further format and images processing, and MATLAB was used to analyze fusion events as previously described (57 (link)).

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Vidal Ceballos A., Díaz A J.A., Preston J.M., Vairamon C., Shen C., Koder R.L, & Elbaum-Garfinkle S. (2022). Liquid to solid transition of elastin condensates. Proceedings of the National Academy of Sciences of the United States of America, 119(37), e2202240119.

Publication 2022

Axio Observer 7 Inverted Microscope Plan-Apochromat Axiocam 506

Frame Immersion Microscope Pluronics f127 Protein

Corresponding Organization :

Other organizations : The Graduate Center, CUNY, CUNY Advanced Science Research Center, City University of New York, Rochester Institute of Technology, City College of New York

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Variable analysis

independent variables

Time of fusion events collection

dependent variables

Fusion events

control variables

Microscope (Axio Observer 7 Inverted Microscope (Zeiss))
Objective (×63/1.4-NA Plan-Apochromat (oil immersion))
Camera (Axiocam 506 mono camera (Zeiss))
Software (Zen software (Zeiss))
Image processing software (ImageJ)
Data analysis software (MATLAB)
Cover glass bottom treatment (1% Pluronics F127 for 25 to 30 min)
Cover glass bottom washing (deionized water)

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