Pathogenic Bacterial Strains for Biofilm Research

Pseudomonas aeruginosa strain PAO1 and S. aureus strain MS2507 were used in this study. Staphylococcus aureus MS2507 was isolated from the blood culture of a patient and was a highly biofilm-forming strain. These strains were cultured in tryptic soy broth (TSB), Luria–Bertani (LB; 1% Bacto tryptone, 0.5% Bacto yeast extract, pH 7.2% and 1.0% NaCl) or on LB agar plates overnight at 37 °C before use.

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Ishihara Y., Hyodo M., Hayakawa Y., Kamegaya T., Yamada K., Okamoto A., Hasegawa T, & Ohta M. (2009). Effect of cyclic bis(3′–5′)diguanylic acid and its analogs on bacterial biofilm formation. Fems Microbiology Letters, 301(2), 193-200.

Publication 2009

Agar Biofilm Culture of blood Nacl Patient Pseudomonas aeruginosa S aureus Staphylococcus aureus Strains Tryptic soy broth Yeast

Corresponding Organization : Nagoya University

Other organizations : Nagoya City University

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Variable analysis

independent variables

Pseudomonas aeruginosa strain PAO1
Staphylococcus aureus strain MS2507

dependent variables

Biofilm formation of Staphylococcus aureus strain MS2507

control variables

Culturing the strains in tryptic soy broth (TSB), Luria–Bertani (LB; 1% Bacto tryptone, 0.5% Bacto yeast extract, pH 7.2% and 1.0% NaCl) or on LB agar plates overnight at 37 °C

controls

No positive or negative controls were explicitly mentioned in the given information.

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