Ovarian Cancer Cell Line Characterization

Human ovarian CCC cell lines RMG1, RMG2, KOC7C, and HAC2 were kindly provided by Dr. H. Itamochi (Tottori University, Tottori, Japan). Human ovarian CCC cell line OVISE, human SAC cell lines SKOV-3 and A2780 were purchased from the American Type Culture Collection. Human ovarian adenocarcinoma cell lines OVCAR4 and OVCAR5 were kindly provided by Cell Culture Facility at Fox Chase Cancer Center (Fox Chase Cancer center, PA, USA). We tested these cells lines in our laboratory for their authentication by morphologic observation. No further cell line authentication was conducted by the authors. Each cell line was never continuously passaged in culture for more than 3 months, and after that, a new vial of frozen cells was thawed. Cells were cultured in DMEM/Ham's F-12, (Gibco, Carlsbad, CA) with 10% fetal bovine serum, as reported previously (15 (link)).