Cholesterol Measurement Protocols

Total serum cholesterol concentration and lipoprotein-cholesterol distribution were analyzed as previously described[12 (link),21 (link)]. Briefly, total serum cholesterol was measured by enzymatic colorimetric method using the Wako Cholesterol E kit (Wako Chemicals USA). Lipoprotein-cholesterol distribution was detected by size exclusion chromatography using a fast performance liquid chromatographic machine (Pharmacia LKB Biotechnology, Uppsala, Sweden).

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Qing H., Liu Y., Zhao Y., Aono J., Jones K.L., Heywood E.B., Howatt D., Binkley C.M., Daugherty A., Liang Y, & Bruemmer D. (2014). Deficiency of the NR4A Orphan Nuclear Receptor NOR1 in Hematopoietic Stem Cells Accelerates Atherosclerosis. Stem cells (Dayton, Ohio), 32(9), 2419-2429.

Publication 2014

Wako Cholesterol E kit

Cholesterol Colorimetric Enzymatic Lipoprotein cholesterol Liquid chromatographic Serum Size exclusion chromatography

Corresponding Organization :

Other organizations : Chongqing Medical University, First Affiliated Hospital of Chongqing Medical University, University of Kentucky, Vanderbilt University

Top 5 similar protocols

Variable analysis

independent variables

Total serum cholesterol concentration
Lipoprotein-cholesterol distribution

dependent variables

Measured outcomes of total serum cholesterol concentration
Measured outcomes of lipoprotein-cholesterol distribution

control variables

None explicitly mentioned

controls

Positive control: None mentioned
Negative control: None mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!