Racemic, (S)-(+)- and (R)-(−)- enantiomers of 13C(6)-labelled PQ (MW 259.1317 g/mol); PQ alcohol, 2-, 3-, and 4-hydroxyprimaquine and CPQ were synthesized as previously reported [9 (link), 22 (link)–24 (link)]. The identity of the compounds synthesized was confirmed by spectral infra-red (IR), nuclear magnetic resonance (NMR), high-resolution mass spectrometry and physical data in comparison with published values.
Co-factors for enzyme activity, including glucose-6-phosphate and its dehydrogenase, reduced nicotinamide adenine dinucleotide phosphate and magnesium chloride were purchased from Sigma-Aldrich (St Louis, MO, USA). Analytical grade solvents, including acetonitrile and methanol were purchased from Fisher Scientific (Fair Lawn, NJ, USA). Milli-Q system (Millipore, Bedford, MA, USA) was used to purify water for analytical utility.
Co-factors for enzyme activity, including glucose-6-phosphate and its dehydrogenase, reduced nicotinamide adenine dinucleotide phosphate and magnesium chloride were purchased from Sigma-Aldrich (St Louis, MO, USA). Analytical grade solvents, including acetonitrile and methanol were purchased from Fisher Scientific (Fair Lawn, NJ, USA). Milli-Q system (Millipore, Bedford, MA, USA) was used to purify water for analytical utility.
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