Protocol detail

Cell Transplantation to Tailbud Embryos

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In order to target cells to the future tailbud, cells from fluorescent dextran labeled (either Rhodamine, Fluorescein, or Cascade Blue dextran, MW 10,000, Molecular Probes) sphere stage donor embryos were transplanted into the ventral margin of unlabeled shield stage host embryos, as previously described (Martin and Kimelman, 2012 (link)). Transplantations were performed under a Leica S6E dissecting microscope using a Cell Tram Vario (Eppendorf). Statistical analysis of quantified cell transplants was performed with the Fisher’s exact test.

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Row R.H., Pegg A., Kinney B.A., Farr GH 3.r.d., Maves L., Lowell S., Wilson V, & Martin B.L. (2018). BMP and FGF signaling interact to pattern mesoderm by controlling basic helix-loop-helix transcription factor activity. eLife, 7, e31018.

Publication 2018

Rhodamine Fluorescein Cascade Blue dextran Cell Tram Vario

Blue dextran Cell Cell transplants Dextran Donor Embryos Fluorescein Microscope Molecular probes Rhodamine Transplantations

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Variable analysis

independent variables

Transplantation of cells from fluorescent dextran labeled (Rhodamine, Fluorescein, or Cascade Blue dextran, MW 10,000, Molecular Probes) sphere stage donor embryos into the ventral margin of unlabeled shield stage host embryos

dependent variables

Not explicitly mentioned

control variables

Cell transplantations performed under a Leica S6E dissecting microscope using a Cell Tram Vario (Eppendorf)
Sphere stage donor embryos labeled with fluorescent dextran (Rhodamine, Fluorescein, or Cascade Blue dextran, MW 10,000, Molecular Probes)

controls

Unlabeled shield stage host embryos

Annotations

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