Evaluating NDH-4338 Wound Healing Efficacy

To ensure this model is useful for therapeutic evaluation, a wound healing efficacy study was performed using a formulation known to treat NM wounds in mice.²⁰ The DDD model was then validated using a test formulation, NDH‐4338 in 49.5% PEG400 and 49.5% lanolin, that was previously shown to treat NM burns and reduce expression of associated markers such as iNOS, COX‐2 and mast cell degranulation.²⁰ Optimized DDD model parameters were used for this study. One hour after NM application, mice were anesthetized and 20 μl of NDH‐4338 formulation or vehicle only were applied to the vesicant exposed regions. Mice were then kept unconscious for 5 min before being monitored while recovering from anesthesia. The treatment was administered every 6 h for 24 h (QID), at which point mice were euthanized and edema was evaluated.