Polyacrylamide Gel Fabrication and Characterization

Fibronectin-coated (5 μg/ml, BD Biosciences, Franklin, Lakes, NJ, USA) polyacrylamide gels were prepared on glass slides (Fisher, Waltham, MA, USA) as previously described22 (link). Two ratios of acrylamide to bis-acrylamide (Fisher), 50:1 and 12.5:1, were used to make gels with Young’s moduli of 1 kPa and 308 kPa, respectively. The Young’s moduli of the polyacrylamide gels were measured using a rheometer as previously described22 (link).

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Alam S.G., Zhang Q., Prasad N., Li Y., Chamala S., Kuchibhotla R., KC B., Aggarwal V., Shrestha S., Jones A.L., Levy S.E., Roux K.J., Nickerson J.A, & Lele T.P. (2016). The mammalian LINC complex regulates genome transcriptional responses to substrate rigidity. Scientific Reports, 6, 38063.

Publication 2016

Fibronectin-coated glass slides acrylamide bis-acrylamide

Acrylamide Fibronectin Gels Polyacrylamide gels

Corresponding Organization :

Other organizations : University of Florida, HudsonAlpha Institute for Biotechnology, Cancer Genetics (United States), Sanford Research, University of Massachusetts Chan Medical School

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Variable analysis

independent variables

Ratio of acrylamide to bis-acrylamide (50:1 and 12.5:1)

dependent variables

Young's moduli of the polyacrylamide gels (1 kPa and 308 kPa)

control variables

Fibronectin-coated (5 μg/ml, BD Biosciences, Franklin, Lakes, NJ, USA) polyacrylamide gels prepared on glass slides (Fisher, Waltham, MA, USA)
The Young's moduli of the polyacrylamide gels were measured using a rheometer as previously described22 (link)

controls

The Young's moduli of the polyacrylamide gels were measured using a rheometer as previously described22 (link)

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