Dominant Negative (DN)-RhoA (plasmid 15901) and constitutively active (CA)-RhoA (plasmid 15900) (Nobes & Hall 1999 (link)) were obtained from Addgene (Cambridge, MA). pTagRFP-N was acquired from Evrogen (Moscow, Russia). pEGFP-Actin was obtained from Clontech (Mountain View, CA).
Protocol detail
Imaging Dominant Negative and Constitutively Active RhoA
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Actin
Plasmid
Rhoa
Corresponding Organization :
Other organizations : University of Minnesota Medical Center
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Variable analysis
independent variables
- Dominant Negative (DN)-RhoA
- Constitutively Active (CA)-RhoA
- Unspecified
- PTagRFP-N
- PEGFP-Actin
- Positive control: pTagRFP-N, pEGFP-Actin
- Negative control: Not explicitly mentioned
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