Protocol detail

Murine Skin Grafting and Tissue Analysis

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The procedure was performed as previously described12 (link). Briefly, after shaving the corresponding lateral aspects of each mouse, matching skin incisions were made from behind the ear to the tail of each mouse, and the subcutaneous fascia was bluntly dissected to create about 0.5 cm of free skin. The scapulas were sutured using a mono-nylon 5.0 (Ethicon, Albuquerque, NM), and the dorsal and ventral skins were approximated by continuous suture. Mice were joined for 4 weeks, and thymus and spleen were then collected and analyzed as described above.

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Tacke R., Hilgendorf I., Garner H., Waterborg C., Park K., Nowyhed H., Hanna R.N., Wu R., Swirski F.K., Geissmann F, & Hedrick C.C. (2015). The transcription factor NR4A1 is essential for the development of a novel macrophage subset in the thymus. Scientific Reports, 5, 10055.

Publication 2015

mono-nylon 5.0

Fascia Mice Nylon Scapulas Skins Spleen Suture Tail Thymus

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Variable analysis

independent variables

Duration of mice joining (4 weeks)

dependent variables

Thymus and spleen collection and analysis

control variables

Shaving of corresponding lateral aspects of each mouse
Skin incisions from behind the ear to the tail of each mouse
Blunt dissection of subcutaneous fascia to create about 0.5 cm of free skin
Suturing of scapulas using mono-nylon 5.0
Approximation of dorsal and ventral skins by continuous suture

controls

Positive control: Not mentioned
Negative control: Not mentioned

Annotations

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