Protocol detail

Quantitative Liver Organoid Analysis

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Albumin production in 10 μL samples of LO media was measured using an enzyme linked immunosorbent assay (Human Albumin ELISA Quantitation Set, Bethyl laboratories Inc, Montgomery, TX) as per manufacturer’s instructions. Urea secretion was measured using the urease based colorimetric method described in Zawada et al. [17 (link)]. Glucose concentration in 10 μL samples was determined by the Yellow Springs Glucose 2300 STAT as per manufacturer’s instructions. Complete liver organoid growth medium (i.e. upcyte® Hepatocyte Growth Medium and upcyte® LSEC Growth Medium in 1:1 ratio) including all the adjuncts (e.g. FBS), was used as a blank for all readings.

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Mattei G., Magliaro C., Giusti S., Ramachandran S.D., Heinz S., Braspenning J, & Ahluwalia A. (2017). On the adhesion-cohesion balance and oxygen consumption characteristics of liver organoids. PLoS ONE, 12(3), e0173206.

Publication 2017

Human Albumin ELISA Quantitation Set

Albumin Colorimetric Elisa Glucose Growth medium Hepatocyte Human albumin Liver Organoid Secretion Springs Urea Urease

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Variable analysis

independent variables

None specified

dependent variables

Albumin production
Urea secretion
Glucose concentration

control variables

Complete liver organoid growth medium (i.e. upcyte® Hepatocyte Growth Medium and upcyte® LSEC Growth Medium in 1:1 ratio) including all the adjuncts (e.g. FBS)

controls

Complete liver organoid growth medium (i.e. upcyte® Hepatocyte Growth Medium and upcyte® LSEC Growth Medium in 1:1 ratio) including all the adjuncts (e.g. FBS) was used as a blank for all readings.

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